Product Name Anti-c-Jun (Phospho-Thr91) Antibody (43021)
Description Anti-c-Jun (Phospho-Thr91) Rabbit Polyclonal Antibody
Target c-Jun (Phospho-Thr91)
Species Reactivity Human, Mouse, Rat
Immunogen Peptide sequence that includes phosphorylation site of threonine 91 (T-T-T(p)-P-T) derived from human c-Jun and conjugated to KLH.
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Purification Purified by Protein A affinity chromatography
Specificity This antibody detects endogenous human, mouse, and rat c-Jun only when phosphorylated at threonine 91.
Target ID c-Jun (Phospho-Thr91)
Post Translational Modification c-Jun (Phospho-Thr91)
Research Areas Phosphospecific Antibodies
Background c-Jun in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function
Storage This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,Serotype000. A band of ~43kDa is detected.Immunohistochemistry: use at dilution of 1:50- 1:100.These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.