Bovine Serum Albumin High Sensitivity ELISA Kit (ECP7816)
$443.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Bovine |  |
Overview
Product Name Bovine Serum Albumin High Sensitivity ELISA Kit (ECP7816)
Description Bovine Serum Albumin ELISA Kit (ECP7816) is a Competitive Inhibition High Sensitivity ELISA for the quantitiation of Bovine Serum Albumin in a variety of aqueous sample types. The high sensitivity kit offsets a reduced dynamic range for greater sensitivity compared to the standard kit.
Target Bovine Serum Albumin
Species Reactivity Bovine
Assay Type Competitive Inhibition
Applications ELISA
Properties
Background Minimum Level of Detection: 87.2 ng/mL
Dynamic range of quantitation: 246.9-20,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 246.9-20,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Bovine Serum Albumin Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Bovine Serum Albumin Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Bovine Serum Albumin
Uniprot ID P02769
Alternative Names BSA
Research Areas Biochemicals
Background Binds water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.