BNIP3 Polyclonal Antibody
Catalog No.: 2290
Format: 100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
BNIP3 is a member of the apoptotic Bcl-2 protein family that is involved in both necrosis and apoptosis. Humans and other animals, as well as lower eukaryotes, encode several BNIP3 paralogues including human BNIP3L which induces apoptosis by interacting with viral and cellular anti-apoptosis proteins. BNIP3 interacts with the E1B 19kDa protein, which is responsible for the protection of virally induced cell death, as well as with E1B-like sequences of BCL2 which is also an apoptotic protector. The BNIP3 gene contains a BH3 domain and a transmembrane domain that have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by the BNIP3 gene is known to induce apoptosis, even in the presence of BCL2.
Antigen: Synthetic peptide corresponding to amino acids at the C-terminus of human BNIP3.
Accession no.: NP_004043.3, Q12983
Gene ID: 664
Host Species: Rabbit
Specificity: This antibody recognizes human and mouse BNIP3.
Immunoblotting: use at 1ug/ml. A band of ~30kDa is detected; an additional ~60kDa band, complexed BNIP3, may also be detected.
Immunofluorescence: use at 10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.