Anti-Bmf (NT) Antibody
Catalog No.: 11002
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Antigen: Peptide corresponding to aa 2-14 of human Bmf (accession no. NP_277038).
Host Species: Rabbit
Preservatives: 0.02% sodium azide
This antibody recognizes an epitope at the N-terminus of human Bmf (Bcl-2-Modifying Factor), a novel BH3-only protein. In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell’s actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiaing the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Immunoblotting: use at 2ug/ml. A band of 25kDa is detected.
Immunocytochemistry: use at 10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentration for their application.
Positive control: HepG2 or 293 cell lysates.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.