Anti-BK β3a K+ Channel Antibody (11528)
$436.00
SKU: 11528
Categories: Antibody Products, Ion Channel Antibodies, Products
Overview
Product Name Anti-BK β3a K+ Channel Antibody (11528)
Description Anti-BK β3a K+ Channel Mouse Monoclonal Antibody
Target BK β3a K+ Channel
Species Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone ID S40B-18
Isotype IgG1
Immunogen Fusion protein corresponding to aa 1-49 (unique N-terminus) of mouse BK Beta3a (accession no. XP_989315). This sequence is 86
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat BK Beta3a. It does not react with BK Beta1, Beta2, Beta3b or Beta4.
Target Name Calcium-activated potassium channel subunit β-3
Target ID BK β3a K+ Channel
Uniprot ID Q9NPA1
Alternative Names BK channel subunit β-3, BKβ3, Hβ3, Calcium-activated potassium channel, subfamily M subunit β-3, Charybdotoxin receptor subunit β-3, K(VCAβ-3, Maxi K channel subunit β-3, Slo-β-3
Gene Name KCNMB3
Accession Number NP_055222.3
Sequence Location Membrane; Multi-pass membrane protein.
Biological Function Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. {PubMed:10766764, PubMed:10864947}.
Research Areas Ion Channels
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. BK channels contribute to electrical impulses, proper signal transmission, and regulation of neurotransmitter release. Research suggests that distribution of the beta subunits in the brain can modulate the BK channels to contribute to the patho- physiology of epilepsy and dyskinesia.
Application Images
Description Immunoblotting: use at 1ug/ml. A band of ~32kDa is detected. Lysate of COS cells transiently-transfected with BK Beta3a plasmid and probed with #11528.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~32kDa is detected.Immunohistochemistry and
Immunocytochemistry: use at 0.1-1ug/mL
Immunofluorescence: use at 1-10ug/mL
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Adult rat brain lysate
Immunocytochemistry: use at 0.1-1ug/mL
Immunofluorescence: use at 1-10ug/mL
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Adult rat brain lysate
References & Data Sheet
Data Sheet Download PDF Data Sheet