Biliverdin Reductase Polyclonal Antibody
Catalog no.: 56257-100
Format: 100ug Protein A-purified antibody in PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
In mammalian species, biliverdin reductase (BVR) converts tetrapyrrole to bilirubin. BVR is a unique enzyme in that it has two pH optima (6.8 and 8.7) and uses different cofactors at each pH optimum: NADH at pH 6.8 and NADPH at pH 8.7. BVR displays pI and molecular mass microheterogeneity which are likely a result of post-translational modifications. BVR is not inactivated by heat shock, and its microheterogeniety is preserved under thermal stress. Human BVR is a longer polypeptide than the rat equivalent (41-42kDa vs. 33kDa), although human and rat BVR share some epitopes and show immunochemical cross-reactivity.
Antigen: Recombinant rat liver biliverdin reductase.
Accession no.: P46844
Gene ID: 116599
Host Species: Rabbit
Antibody Class: Polyclonal
Specificity: This antibody recognizes human, mouse, rat, porcine, and hamster biliverdin reductase.
Immunoblotting: Use at 1-5ug/ml. A band of ~33kDa (rat) and ~41-42kDa (human) is detected.
Immunohistochemistry: use at 1-10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their application.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze/thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures