Beta-Lactamase Monoclonal Antibody
Catalog No.: 15721
Size: 100ug or 500ug PEG-purified antibody in PBS, pH 7.4.
The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to microbial production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta- lactam bond which destroys anti-bacterial activity. Bacteria the produce TEM- or SHV-type beta- lactamases have point mutations in structural genes that have extended the substrate specificity of these beta-lactamases. As a result, many of the beta-lactamase-producing Gram-negative bacteria have become multi-drug resistant. Beta-lactamase from Enterobacter cloacae is a serine beta-lactamase with substrate specificity for cephalosporins.
Antigen: Type IV beta lactamase from Enterobacter cloacae. Predicted molecular wt. ~41kDa.
MMRKSLCCAL LLGISCSALA TPVSEKQLAE VVANTITPLM KAQSVPGMAV AVIYQGKPHY YTFGKADIAA NKPVTPQTLF ELGSISKTFT GVLGGDAIAR GEISLDDAVT RYWPQLTGKQ WQGIRMLDLA TYTAGGLPLQ VPDEVTDNAS LLRFYQNWQP QWKPGTTRLY ANASIGLFGA LAVKPSGMPY EQAMTTRVLK PLKLDHTWIN VPKAEEAHYA WGYRDGKAVR VSPGMLDAQA YGVKTNVQDM ANWVMANMAP ENVADASLKQ GIALAQSRYW RIGSMYQGLG WEMLNWPVEA NTVVEGSDSK VALAPLPVAE VNPPAPPVKA SWVHKTGSTG GFGSYVAFIP EKQIGIVMLA NTSYPNPARV EAAYHILEAL Q
Accession no.: P05364
Host Species: Mouse
Clone no.: 3E11.G3
Antibody Subtype: IgG3
Specificity: This antibody recognizes Type IV beta-lactamase of Enterobacter cloacae.
ELISA: use at 10-20ug/ml (optimized for immobilized beta-lactamase at 10ug/ml). Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This product is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.