Anti-β 3 GABA-A Receptor Antibody (56482)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,AM Human, Mouse, Rat
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Overview
Product Name Anti-β 3 GABA-A Receptor Antibody (56482)
Description Anti-β 3 GABA-A Receptor Mouse Monoclonal Antibody
Target β 3 GABA-A Receptor
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID S87-25
Isotype IgG1
Immunogen Fusion protein corresponding to aa 370-433 of mouse Beta 3 GABA-A receptor (accession no. AAB60502).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Beta 3 GABA-A receptor. It does not cross-react with Beta 1 or 2 GABA-A receptor.
Target Name γ-aminobutyric acid receptor subunit β-3
Target ID β 3 GABA-A Receptor
Uniprot ID P63080
Alternative Names GABA(A receptor subunit β-3
Gene Name Gabrb3
Sequence Location Cell junction, synapse, postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane
Biological Function Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447). {UniProtKB:P28472, PubMed:10670447, PubMed:27129275, PubMed:9108119}.
Research Areas Neuroscience
Background Ion channels are integral membrane proteins thathelp establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. In some ion channels, the flow of ions is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. The GABA-A receptor is a fast acting ligand-gated ion channel. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (56482). Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56482) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (56482). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56482) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25 (56482). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody (56482) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.

Immunohistochemistry: use at 1-10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain lysate.
References & Data Sheet