Beclin 1 Polyclonal Antibody
Catalog No. Size
Format: Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Beclin 1, the mammalian orthologue of yeast Atg6, has a central role in autophagy. It interacts with several cofactors (Atg14L, UVRAG, Bif-1, Rubicon, Ambra1, HMGB1, nPIST, VMP1, SLAM, IP3R, PINK and survivin) to regulate the lipid kinase Vps-34 protein and promote formation of Beclin 1-Vps34-Vps15 core complexes, thereby inducing autophagy. In contrast, the BH3 domain of Beclin 1 is bound to, and inhibited by Bcl-2 or Bcl-XL. This interaction can be disrupted by phosphorylation of Bcl-2 and Beclin 1, or ubiquitination of Beclin 1. Beclin 1 dysfunction has been implicated in many disorders including cancer and neurodegeneration.
Antigen: Synthetic peptide corresponding to mid-protein amino acids of human Beclin 1.
Accession no. NP_001300927.1 Gene ID 8678 SwissProt Q14457
Host Species: Rabbit
Specificity: This antibody recognizes human and rat Beclin 1.
Immunoblotting: use at 1- 2ug/ml. A band of ~51kDa is detected.
Immunofluorescence: use at 10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.