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Anti-Bcl-xL (Phospho-Ser62) Antibody (43066)

$355.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IF Human, Mouse, Rat
SKU: 43066 Categories: ,
Overview
Product Name Anti-Bcl-xL (Phospho-Ser62) Antibody (43066)
Description Anti-Bcl-xL (Phospho-Ser62) Rabbit Polyclonal Antibody
Target Bcl-xL (Phospho-Ser62)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 62 derived from human Bcl-xL and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat Bcl-xL only when phosphorylated at serine 62.Accession no.: NP_612815.1
Target ID Bcl-xL (Phospho-Ser62)
Post Translational Modification Bcl-xL (Phospho-Ser62)
Target Phospho-ser62
Research Areas Phosphospecific Antibodies
Background B-cell lymphoma-extra large (Bcl-xl, or BCL2-like 1 isoform 1) is a transmembrane protein in the mitochondria. It is a member of the Bcl-2 family of proteins and acts as a pro-survival protein by preventing the release of mitochondrial cytochrome c, which would lead to caspase activation. Relative amounts of pro- and anti-survival Bcl-2 family proteins define whether a cell will undergo cell death: if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A ban of ~30kDa is detected.

Immunohistochemistry: use at dilution of 1:50-1:100.

Immunofluorescence: use at dilution of 1:100-1:200.

These are recommended working dilutions.

End user should determine optimal dilutions for their application.
References & Data Sheet