Anti-Bcl-3 Monoclonal Antibody
Catalog No.: 11067 (clone 150-3.5)
Size: 100ug IgG in PBS, pH 7.4, purified by Protein G affinity chromatography.
The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the IκB subfamily of inhibitors of NFκB transcription factors. Bcl- 3 does not have DNA-binding activity, but it interacts with NFκB1 and NFκB2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NFκB family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney, and testes.
Immunogen: Recombinant mouse Bcl-3- 6His.
Host Species: Hamster
Preservatives: None. Available on request.
This antibody recognizes human and mouse Bcl-3.
Immunoblotting: use at 2ug/ml. A band of approx. 64kDa is detected.
ELISA: Enduser should determine optimal concentration for each sample.
Positive control: Jurkat T cell lysate.
See Bassetti MFJ et al. 2009 International Immunology 21: 339-348.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.