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Anti-BAD (Phospho-Ser112) Antibody (43067)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse
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Overview
Product Name Anti-BAD (Phospho-Ser112) Antibody (43067)
Description Anti-BAD (Phospho-Ser112) Rabbit Polyclonal Antibody
Target BAD (Phospho-Ser112)
Species Reactivity Human, Mouse
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 112 (H-S-S(p)-Y-P) derived from mouse BAD and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human and mouse BAD only when phosphorylated at serine 112.Accession no.: Q61337, NP_031548.1
Target Name Bcl2-associated agonist of cell death
Target ID BAD (Phospho-Ser112)
Uniprot ID Q92934
Gene Name BAD
Gene ID 603167
Post Translational Modification BAD (Phospho-Ser112)
Target Phospho-ser112
Sequence Location Mitochondrion outer membrane. Cytoplasm
Biological Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. {ECO:0000250}.
Research Areas Phosphospecific Antibodies
Background The protein encoded by the BAD gene is a member of the Bcl-2 family whose members are regulators of programmed cell death. BAD protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2 and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin, were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500- 1:1,000. A ban of ~23kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their application.
References & Data Sheet