Bacillus anthracis Protective Antigen

Bacillus anthracis Protective Antigen Monoclonal Antibody

ORDERING INFORMATION

Catalog Nos.: 18720 (C3), 18721 (101), 18722 (102), 18723 (103), 18724 (104), 18725 (105), and 18726 (106).
Size: 200 ug purified antibody in PBS, pH 7.4.

BACKGROUND

Anthrax toxin produced by Bacillus anthracis is composed of three proteins: Protective Antigen (PA), Edema Factor (EF), and Lethal Factor (LF). Toxicity requires the assembly of all three of these proteins, and this process is initiated when full-length PA binds to the cell surface ATR (anthrax toxin receptor). After binding, PA is enzymatically cleaved to yield a 63 kD fragment which then binds EF and LF, and the complex is internalized.

SPECIFICATION SUMMARY

Antigen: Purified B. anthracis Protective Antigen (83 kD).
Host Species: Mouse
Antibody Class: IgG1=18720, 18725, 18726; IgG2b=18721, 18722, 18723, 18724
Preservative: 0.1% NaN3

SPECIFICITY

These antibodies recognize different epitopes of the 83kD and 63 kD forms of B. anthracis PA. They do not cross-react with B. anthracis EF, LF, or spore/vegetative surface antigens.

APPLICATIONS

Immunoblotting: use 18721, 18723, 18725, or 18726 at 5 ug/ml.
Sandwich ELISA: use coating antibody (18720 or 18725) at 5 ug/ml; use detect antibody (18722, 18723, or18726) at 1 ug/ml.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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