Bacillus anthracis Protective Antigen Monoclonal Antibody
Catalog Nos.: 18720 (C3), 18721 (101), 18722 (102), 18723 (103), 18724 (104), 18725 (105), and 18726 (106).
Size: 200 ug purified antibody in PBS, pH 7.4.
Anthrax toxin produced by Bacillus anthracis is composed of three proteins: Protective Antigen (PA), Edema Factor (EF), and Lethal Factor (LF). Toxicity requires the assembly of all three of these proteins, and this process is initiated when full-length PA binds to the cell surface ATR (anthrax toxin receptor). After binding, PA is enzymatically cleaved to yield a 63 kD fragment which then binds EF and LF, and the complex is internalized.
Antigen: Purified B. anthracis Protective Antigen (83 kD).
Host Species: Mouse
Antibody Class: IgG1=18720, 18725, 18726; IgG2b=18721, 18722, 18723, 18724
Preservative: 0.1% NaN3
These antibodies recognize different epitopes of the 83kD and 63 kD forms of B. anthracis PA. They do not cross-react with B. anthracis EF, LF, or spore/vegetative surface antigens.
Immunoblotting: use 18721, 18723, 18725, or 18726 at 5 ug/ml.
Sandwich ELISA: use coating antibody (18720 or 18725) at 5 ug/ml; use detect antibody (18722, 18723, or18726) at 1 ug/ml.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.