Bacillus anthracis Lethal Factor Antigen Monoclonal Antibody
Catalog Nos.: 18729 (105) and 18730 (106).
Size: 200 ug purified antibody in PBS, pH 7.4.
Anthrax toxin produced by Bacillus anthracis is composed of three proteins: Protective Antigen (PA), Edema Factor (EF), and Lethal Factor (LF). Toxicity requires the assembly of all three of these proteins, and this process is initiated when PA binds to the cell surface ATR (anthrax toxin receptor). After binding, PA is enzymaticaly cleaved to yield a 63 kD fragment which then binds EF and LF, and the complex is internalized. LF is a protease and cleaves enzymes of the MAPKK (mitogen-activated protein kinase kinase) family.
Antigen: Purified B. anthracis Lethal Factor (82 kD).
Host Species: Mouse
Antibody Class: IgG1 Preservative: 0.1% NaN3
These antibodies recognize different epitopes of B. anthracis LF. They do not cross-react with B. anthracis PA, EF, or spore/vegetative surface antigens.
Immunoblotting: use at 5 ug/ml.
Sandwich ELISA: use coating antibody (18730) at 5 ug/ml; use detect antibody (18729) at 1 ug/ml.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.