Product Name Anti-ATR Antibody (13016)
Description Anti-ATR Rabbit Polyclonal Antibody
Species Reactivity Human, Mouse
Isotype Whole IgG
Immunogen Synthetic peptide representing a portion of the protein encoded within exon 5 (LocusLink ID 545).
Concentration Lot Specific
Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Buffer Formulation Tris
Buffer pH pH 7.8
Buffer Anti-Microbial 0.1% Sodium Azide
Purification Purified by immunoaffinity chromatography
Specificity This antibody reacts with human and mouse. Antibody is affinity purified.
Target Name Serine/threonine-protein kinase ATR
Target ID ATR
Uniprot ID Q13535
Alternative Names EC 18.104.22.168, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1
Gene Name ATR
Accession Number NP_001175
Sequence Location Nucleus, Chromosome
Biological Function Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. RUniProtKB:Q9JKK8, PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11114888, PubMed:11418864, PubMed:11673449, PubMed:11721054, PubMed:11865061, PubMed:12526805, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15314022, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169, PubMed:9925639}.
Research Areas Enzymes
Description Detection of human ATR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATR antibody (13016 lot 3) used for IP at 6 µg per reaction. ATR was also immunoprecipitated by a previous lot of this antibody (13016 lot 2) and a second antibody against a different epitope of rabbit ATR (13018). For blotting immunoprecipitated ATR, 13016 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Description Detection of human ATR by western blot. Samples: Whole cell lysate (10 µg) from U2OS, HeLa, MCF-7, RKO, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody (13016 lot 3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Storage Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Western Blot: 0.033 - 0.067 ug/ml.