Anti-Atg5 Antibody

$436.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human
SKU: 23005 Categories: , ,
Overview
Product Name Anti-Atg5 Antibody (23005)
Description Anti-Atg5 Rabbit Polyclonal Antibody
Target Atg5
Species Reactivity Human
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids at the N-terminus of human Atg5.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human Atg5.
Target Name Autophagy protein 5
Target ID Atg5
Uniprot ID Q9H1Y0
Alternative Names APG5-likeApoptosis-specific protein
Gene Name ATG5
Sequence Location Cytoplasm, Preautophagosomal structure membrane; Peripheral membrane protein. Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Biological Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. {UniProtKB:Q99J83, PubMed:12207896, PubMed:20580051, PubMed:22170153, PubMed:26812546}.; May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. {PubMed:15778222, PubMed:7796880}.; (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601). {PubMed:17709747, PubMed:19666601}.
Research Areas Autophagy
Background Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. The protein encoded by the Atg5 gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene.
Application Images
Description Immunoblotting: use at 1-2ug/ml. A band of ~50kDa, corresponding to Atg12-Atg5 complex, is detected. Note: the predicted molecular weight of ATG5 is ~32kDa. Detection of Atg5- ATG12 in 20ug of HeLa cell lysate.
Description Immunofluorescence: use at 10ug/ml. Detection of Atg5 in formaldehyde-fixed HeLa cells.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~50kDa, corresponding to Atg12-Atg5 complex, is detected. Note: the predicted molecular weight of ATG5 is ~32kDa.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet