Atg4C Polyclonal Antibody
Catalog No. Size
Format: Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg4C is a homolog of yeast Apg4, a cysteine protease involved in autophagy. The deduced 458-amino acid protein contains a putative active-site cysteine at position 110. Atg4C shares significant similarity with yeast Apg4, except for divergence at its N- and C-terminal ends. The absence of an N-terminal signal sequence suggests that Atg4C is a cytoplasmic enzyme. Northern blot analysis detected a 3.5-kb transcript in many tissues, with highest expression in skeletal muscle, heart, liver, and testis. It was also detected in some tumor cell lines, but not in fetal tissues.
Antigen: Synthetic peptide corresponding to amino acids at the N-terminus of human Atg4C.
Accession no. NP_116241.2 Gene ID 84938 SwissProt Q96DT6
Host Species: Rabbit
Specificity: This antibody recognizes human and rat Atg4C.
Immunoblotting: use at 1-2ug/ml. A band of ~52kDa is detected.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.