Anti-Atg2A Antibody (23001)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human
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Overview
Product Name Anti-Atg2A Antibody (23001)
Description Anti-Atg2A Rabbit Polyclonal Antibody
Target Atg2A
Species Reactivity Human
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids at the C-terminus of human ATG2A.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human Atg2A.
Target Name Autophagy-related protein 2 homolog A
Target ID Atg2A
Uniprot ID Q2TAZ0
Gene Name ATG2A
Sequence Location Preautophagosomal structure membrane, Lipid droplet, Endoplasmic reticulum membrane
Biological Function Lipid transfer protein involved in autophagosome assembly (PubMed:28561066, PubMed:31271352, PubMed:30952800). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed:31271352, PubMed:30952800). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PubMed:31271352, PubMed:30952800). Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Also regulates lipid droplets morphology and distribution within the cell (PubMed:22219374, PubMed:28561066). {PubMed:22219374, PubMed:28561066, PubMed:30952800, PubMed:31271352}.
Research Areas Autophagy
Background Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Two human Atg2 homologs (Atg2A, Atg2B) are critical for autophagosome formation as silencing of both results in the accumulation of unclosed autophagic structures. Starvation-induced autophagy targets Atg2A to the initiation site of autophagosome biogenesis, where it associates with DFCP1, WIPI-1, and other autophagy- related proteins. Atg2 proteins also function in lipid droplet metabolism as depletion of both Atg2A and AtgB results in changes in the size, number, and distribution of lipid droplets. An increase in Atg2A expression during etoposide- and doxorubicin-induced apoptosis suggests that Atg2A may be a useful indicator of topoisomerase II inhibitor-mediated apoptosis.
Application Images
Description Immunoblotting: use at 1-2ug/ml. A band of ~213kDa is detected. Detection of Atg2A in 20ug HeLa cell lysate.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~213kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet