ATF2 (Phospho-Thr73 or 55) Polyclonal Antibody
Catalog No.: 43032
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
ATF2 is a transcription factor that is a member of the leucine zipper family of DNA binding protein that can perform multiple functions. It can bind to the cAMP-responsive element (CRE), and it can form a homodimer or a heterodimer with c-Jun and stimulate CRE-dependent transcription. ATF2 is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro. ATF2 may also be involved in a cell’s DNA damage response independent of its role in transcriptional regulation.
Antigen: Peptide sequence that includes phosphorylation sites of threonine 73 or 55 (T-P-T(p)- R-F) derived from human ATF2 and conjugated to KLH.
Accession no.: P15336, NP_001871.2
Host Species: Rabbit
This antibody detects endogenous human, mouse, rat, and monkey ATF2 only when phosphorylated at threonine 73 or 55.
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~65-75kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100.
Immunofluorescence: use at 1:100-1:200.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term use.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.