ASIC1 Monoclonal Antibody
Catalog No.: 11573 (clone S271-44)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Acid sensing (proton-gated) ion channel 1 (ASIC1) is a cation channel with high affinity for sodium which is gated by extracellular protons and inhibited by the diuretic amiloride. It is also permeable for Ca(2+), Li(+) and K(+) and mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. ASIC1 functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin- dependent protein kinase II phosphory- lation.
Antigen: Fusion protein corresponding to aa 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (accession no. Q6NXK8).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes mouse and rat ASIC1. It does not cross-react with ASIC2.
Immunoblotting: use at 1ug/ml. A band of ~60kDa is detected.
Positive control: Rat brain lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.