Anti-Apaf1 (NT) Antibody
Catalog No.: 2412
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease- activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Antigen: Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse.
Host Species: Rabbit
Preservatives: 0.02% sodium azide
This antibody recognizes full-length mouse and human Apaf1 (115-130kDa).
Immunoblotting: use at 1-2ug/ml
Immunohistochemistry: use at 1-2ug/ml
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Positive control: Whole cell lysate of HeLa or K562 cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.