Amyloid Beta 1-40 (Aβ40) Monoclonal Antibody
Catalog No.: 57002 (clone Ab40.1)
Size: 100ug in PBS, pH 7.4. Purified by Protein G affinity chromatography.
Accumulation and aggregation of amyloid β (Aβ) in the brain is indicated as the trigger of a pathological cascade that causes Alzheimer disease (AD). The highly amyloidogenic 42-amino acid form of Aβ (Aβ42) and aminoterminally truncated forms of Aβ (Aβx-42) are the predominant species of Aβ typically found in diffuse and senile plaques within the AD brain. There is a strong correlation between Aβ40 positivity and mature plaques. In the AD cortex, diffuse plaques, representing the earliest stage of Aβ deposition, are positive for Aβ42, but negative for Aβ40.
Antigen: Synthetic peptide correspond- ing to Aβ35-40.
Host Species: Mouse
Antibody Class: IgG1
This antibody specifically recognizes an epitope within Aβx-40. NOTE: When administered to young Tg2576 mice with minimal Aβ deposition, this antibody reduced Aβ accumulation in the brain.
Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, Test at 1-10ug/ml.
Sandwich ELISA (works equally well as capture or detection antibody).
These are recommended concentrations; enduser should determine optimal concentrations for their applications.
Sandwich ELISA protocol on next page. See specific product references below for more information.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C.
Levites Y et al. 2006. Anti-Aβ42 and Anti-Aβ40 specific monoclonal antibodies attenuate amyloid deposition in an Alzheimer’s disease mouse model. J Clin Invest 116: 193-201.
Levites Y et al. 2006. Intracranial Adeno-Associated Virus-Mediated Delivery of Anti-Pan Amyloid β, Amyloidβ40, and Amyloid β42 Single-Chain Variable Fragments Attenuates Plaque Pathology in Amyloid Precursor Protein Mice. J Neurosci 26: 11923-11928.
Levites Y et al. 2006. Insights into the mechanisms of action of anti-Aβ antibodies in Alzheimer’s disease mouse models. FASEB J 20: 2576-8.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.
SANDWICH ELISA PROTOCOL
96-well ELISA plates (Immulon HBX4) are coated with capture MAb at 2.5-5ug/well and incubated at 4°C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4°C overnight. The next day plates are washed in PBS and serial dilution of Aβ samples are added; plates are incubated overnight at 4°C. The next day plates are washed in PBS, and HRP-conjugated detection Aβ MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.