Amyloid Beta 1-16 (Aβ1-16) Monoclonal Antibody
Catalog No.: 57003 (clone Ab3)
Size: 100ug in PBS, pH 7.4. Purified by Protein G affinity chromatography.
Accumulation and aggregation of amyloid β (Aβ) in the brain is indicated as the trigger of a pathological cascade that causes Alzheimer disease (AD). There is now compelling evidence that metal binding to Aβ is involved in AD pathogenesis. The amino acid region 1-16 is widely considered as the metal binding domain of Aβ. Unlike copper(II) that prefers the N-terminal amino group as the main binding site, zinc(II) is preferentially placed in the 8-16 amino acidic region of Aβ (1-16).
Antigen: Synthetic peptide correspond- ing to Aβ1-16.
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes Aβ1-16 as well as other Aβ peptides: Aβ37, Aβ38, Aβ39, Aβ40, and Aβ42. NOTE: When administered to young Tg2576 mice with minimal Aβ deposition and to older mice with higher Aβ loads, this antibody reduced Aβ accumulation in the brain.
Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, ELISA.
Test at 1-10ug/ml in all appli- cations. These are recommended concentrations; enduser should determine optimal concentrations for their applications.
See specific product references below for more information.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C.
Levites Y et al. 2006. Anti-Aβ42 and Anti- Aβ40 specific monoclonal antibodies attenuate amyloid deposition in an Alzheimer’s disease mouse model. J Clin Invest 116: 193-201.
Levites Y et al. 2006. Intracranial Adeno- Associated Virus-Mediated Delivery of Anti-Pan Amyloid β, Amyloidβ40, and Amyloid β42 Single- Chain Variable Fragments Attenuates Plaque Pathology in Amyloid Precursor Protein Mice. J Neurosci 26: 11923-11928.
Levites Y et al. 2006. Insights into the mechanisms of action of anti-Aβ antibodies in Alzheimer’s disease mouse models. FASEB J 20: 2576-8.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.