Amyloid Beta 1-16 (Aβ1-16) Monoclonal Antibody
Catalog No.: 57004 (clone Ab5)
Size: 100ug in PBS, pH 7.4. Purified by Protein G affinity chromatography.
Accumulation and aggregation of amyloid β (Aβ) in the brain is indicated as the trigger of a pathological cascade that causes Alzheimer disease (AD). There is now compelling evidence that metal binding to Aβ is involved in AD pathogenesis. The amino acid region 1-16 is widely considered as the metal binding domain of Aβ. Unlike copper(II) that prefers the N-terminal amino group as the main binding site, zinc(II) is preferentially placed in the 8-16 amino acidic region of Aβ (1-16).
Antigen: Aggregated Aβ1-42, fibrillar Aβ.
Host Species: Mouse
Antibody Class: IgG2b
This antibody recognizes an epitope within Aβ1-16 as well as other Aβ peptides: Aβ37, Aβ38, Aβ39, Aβ40, and Aβ42. NOTE: When administered to young Tg2576 mice with minimal Aβ deposition and to older mice with higher Aβ loads, this antibody reduced Aβ accumulation in the brain.
Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, Test at 1-10ug/ml in all applications.
Sandwich ELISA as capture or HRP- conjugated detection antibody.
These are recommended concentrations; enduser should determine optimal concentrations for their applications.
Sandwich ELISA protocol on next page. See specific product references below for more information.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C.
Levites Y et al. 2006. Anti-Aβ42 and Anti-Aβ40 specific monoclonal antibodies attenuate amyloid deposition in an Alzheimer’s disease mouse model. J Clin Invest 116: 193-201.
Levites Y et al. 2006. Intracranial Adeno-Associated Virus-Mediated Delivery of Anti-Pan Amyloid β, Amyloidβ40, and Amyloid β42 Single-Chain Variable Fragments Attenuates Plaque Pathology in Amyloid Precursor Protein Mice. J Neurosci 26: 11923-11928.
Levites Y et al. 2006. Insights into the mechanisms of action of anti-Aβ antibodies in Alzheimer’s disease mouse models. FASEB J 20: 2576-8.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.
SANDWICH ELISA PROTOCOL
96-well ELISA plates are coated with capture MAb at 2.5-5ug/well and incubated at 4°C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4°C overnight. The next day plates are washed in PBS and serial dilution of Aβ samples are added; plates are incubated overnight at 4°C. The next day plates are washed in PBS, and HRP-conjugated detection Aβ MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.