Anti-Alpha 1 GABA-A Receptor Monoclonal Antibody
Catalog No.: 56467 (clone S95-35)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families α, β, γ, and δ have been studied extensively; π and ε have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.
Antigen: Fusion protein corresponding to aa 355-394 of mouse Alpha 1 GABA-A receptor (accession no. P62812).
Host Species: Mouse
Antibody Class: IgG2a
This antibody recognizes human, mouse, and rat Alpha 1 GABA-A receptor. It does not cross-react with Alpha 2 or Alpha 3 GABA-A receptors.
Immunoblotting: use at 1-10ug/ml. A band of ~55kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml
Immunofluorescence: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.