Anti-Akirin 1 Antibody (56264)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB Human, Mouse, Rat
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Overview
Product Name Anti-Akirin 1 Antibody (56264)
Description Anti-Akirin1 Rabbit Polyclonal Antibody
Target Akirin 1
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen N-terminal domain peptide sequence (16 amino acids) derived from human Akirin1 (GenBank accession no. CAI16710).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Akirin1.
Target Name Akirin-1
Target ID Akirin 1
Uniprot ID Q9H9L7
Gene Name AKIRIN1
Sequence Location Nucleus
Biological Function Functions as signal transducer for MSTN during skeletal muscle regeneration and myogenesis. May regulate chemotaxis of both macrophages and myoblasts by reorganising actin cytoskeleton, leading to more efficient lamellipodia formation via a PI3 kinase dependent pathway. {UniProtKB:Q99LF1}.
Research Areas Neuroscience
Background Akirin is a recently discovered nuclear protein with an essential function in the Drosophila melanogaster immune deficiency pathway which responds to gram-negative bacterial infection. Akirin is conserved in vertebrates as at least two genes designated akirin1 and akirin2. In mice, akirin2 functions in toll-lie receptor, tumor necrosis factor, and interleukin-1beta signaling pathways downstream of NF-kappaB to induce the transcription of several immune-response genes. In mice, knockout of akirin1 had no obvious phenotypic effects, but knockout of akirin2 was embryonic-lethal.
Application Images
Description Western blot analysis of Akirin1 in A549 cell lysate with Akirin1 antibody at (A) 1 and (B) 2 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 2ug/mL

Positive control: A549 cell lysate.
References & Data Sheet