Anti-Adenosine deaminase Antibody (13903)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Human
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Overview
Product Name Anti-Adenosine deaminase Antibody (13903)
Description Anti-Adenosine deaminase Mouse Monoclonal Antibody
Target Adenosine deaminase
Species Reactivity Human
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1F5-2G6
Isotype IgG1
Immunogen Native ADA purified from calf spleen and recombinant human ADA.
Associated Products
Associated Antibody Clones

Catalog No.

Clone No.

MAb Subtype

Size

13903

1F5-2G6

IgG1

100ug

13904

2C9-1B4

IgG1

100ug

13905

2F5-1D3

IgG1

100ug

Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity Native and recombinant ADA.
Target Name Adenosine deaminase
Target ID Adenosine deaminase
Uniprot ID P00813
Alternative Names EC 3.5.4.4, Adenosine aminohydrolase
Gene Name ADA
Accession Number NP_000013
Sequence Location Cell membrane, Cytoplasmic vesicle lumen, Cytoplasm, Lysosome
Biological Function Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed:8452534, PubMed:16670267). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). Stimulates plasminogen activation (PubMed:15016824). Plays a role in male fertility (PubMed:21919946, PubMed:26166670). Plays a protective role in early postimplantation embryonic development (By similarity). {UniProtKB:P03958, UniProtKB:P56658, PubMed:11772392, PubMed:15016824, PubMed:16670267, PubMed:20959412, PubMed:21919946, PubMed:23193172, PubMed:26166670, PubMed:8452534}.
Research Areas Enzymes
Background Adenosine deaminase (ADA) is a purine catabolic enzyme which irreversibly deaminates adenosine and deoxyadenosine. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA deficiency (due to mutations in the ADA gene) results in severe combined immunodeficiency (SCID) as a result of accumulation of deoxyadenosine which, in turn, leads to (1) a buildup of dATP which inhibits ribonucleotide reductase and prevents DNA synthesis; since developing T cells and B cells are some of the most mitotically active cells, they are highly susceptible to this condition, and (2) an increase in S- adenosylhomocysteine since ADA is important in the purine salvage pathway; both substances are toxic to immature lymphocytes, which thus fail to mature.
Application Images
Description ELISA: Recombinant human ADA coated on the solid phase at 0.5ug/ml.
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: Recombinant human ADA coated on the solid phase at 0.5ug/ml.

Immunoblotting: use at 200ng/ml. A band of ~38kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet