Anti-Acinus (CP) Antibody
Catalog No.: 2215
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
A new inducer of chromatin condensation was recently identified and designated Acinus (for Apoptotic Chromatin Condensation Inducer in the Nucleus). Acinus is cleaved by caspase-3 and an additional protease to generate a small active peptide, p17, which causes chromatin condensation in vitro when it is added to purfied nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and are designated AcinusL, AcinusS, and AcinusS’.
Antigen: Peptide corresponding to aa 1065-1080 of human AcinusL, aa 307-322 of human Acinus S and aa 338-353 of human AcinusS’ which are identical to those of mouse Acinus. These sequences are located near the C-terminus of the cleaved active peptide p17.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human and mouse Acinus (220 kD).
Immunoblotting: use at 1-10ug/ml
Immunocytochemistry: use at 10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: K562 cells or whole cell lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.