Anti-Acetylated Lysine Antibody

$436.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA All
SKU: 56455 Categories: , ,
Overview
Product Name Anti-Acetylated Lysine Antibody (56455)
Description Anti-Acetylated Lysine Mouse Monoclonal Antibody
Target Acetylated Lysine
Species Reactivity All
Applications WB,IHC,ICC/IF,IP,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 7F8
Isotype IgG1
Immunogen Acetylated KLH
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes proteins containing acetylated lysine residues. It does not react with non-acetylated lysine residues.
Target ID Acetylated Lysine
Research Areas Neuroscience
Background Acetylation is a common post-translational modification of lysine. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Post- translational acetylation and deacetylation of lysine have been recognized as intracellular signals that are critical in regulating gene transcription, cell cycle progression, apoptosis, DNA repair, and cytoskeletal organization. Regulation of protein acetylation is impaired in some cancers and polyglutamine diseases, and HDACs are promising targets for anti-cancer drugs currently under investigation.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
Description Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Acetylated chicken erythrocyte histones.
References & Data Sheet