Anti-Acetylated Lysine Monoclonal Antibody
Catalog No.: 56455 (clone 7F8)
Size: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Acetylation is a common post-translational modification of lysine. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Post- translational acetylation and deacetylation of lysine have been recognized as intracellular signals that are critical in regulating gene transcription, cell cycle progression, apoptosis, DNA repair, and cytoskeletal organization. Regulation of protein acetylation is impaired in some cancers and polyglutamine diseases, and HDACs are promising targets for anti-cancer drugs currently under investigation.
Antigen: Acetylated KLH
Host Species: Mouse
Antibody Class: IgG1
Preservatives: 0.09% sodium azide
Other additives: 50% glycerol
This antibody recognizes proteins containing acetylated lysine residues. It does not react with non-acetylated lysine residues.
Immunoblotting: use at 1ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Acetylated chicken erythrocyte histones.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.