Anti-Acetylated Lysine Antibody (56498)

$470.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF,IP,ELISA All
SKU: 56498 Categories: , , ,
Overview
Product Name Anti-Acetylated Lysine Antibody (56498)
Description Anti-Acetylated Lysine Rabbit Polyclonal Antibody
Target Acetylated Lysine
Species Reactivity All
Applications WB,ICC/IF,IP,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Acetylated lysine-KLH conjugate.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.0 and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.0
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes proteins containing acetylated lysine residues. It does not recognize non-acetylated proteins.
Target ID Acetylated Lysine
CAS Number 692-04-6
PubChem ID 265949
Research Areas Neuroscience
Background Post-translational modifications of proteins play critical roles in the regulation and function of many biological processes. A common post-transcriptional modification of lysine is acetylation. The conserved amino- terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.
Application Images
Description Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498) at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).
Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Description Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (56498) at 1:1000.
Additional Information
Handling
Storage This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mlELISA: use at 1-10ug/ml with acetylated lysine-containing proteins on the solid phase.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet