Anti-6 His Antibody (18814)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 1mg WB,IP,ICC,ELISA 6 His
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SKU: 18814 Categories: , ,
Overview
Product Name Anti-6 His Antibody (18814)
Description Anti-6 His Rabbit Polyclonal Antibody
Target 6 His
Species Reactivity 6 His
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Immunogen 6-His (HHHHHH) conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Target ID 6 His
Research Areas Epitope Tags
Background A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Application Images
Description Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-6-His antibody 18814 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Description Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, rabbit anti-6-His antibody 18814 used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 10 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:20,000.

ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet