6-His Affinity-Purified Polyclonal Antibody
Catalog No., Size
Format: 1mg/ml in Phosphate-Buffered Saline (PBS), pH 7.2, 0.09% sodium azide.
A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Antigen: 6-His (HHHHHH) conjugated to KLH.
Host Species: Rabbit
Specificity: Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Immunoblotting: use at a dilution of 1:1,000-1:20,000.
ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500.
Immunocytochemistry: use at a dilution of 1:100-1:400.
Immunoprecipitation: use at 1-4ug/mg lysate.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at 2-8°C.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.