5-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7784)
$718.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name 5-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7784)
Description 5-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7784) is a Competitive Inhibition ELISA for the quantitiation of 5-Hydroxyeicosatetraenoic Acid in a variety of aqueous sample types.
Target 5-Hydroxyeicosatetraenoic Acid
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 320.473 g·mol−1
Background Minimum Level of Detection: 3.87 ng/mL
Dynamic range of quantitation: 9.88-800 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 9.88-800 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| 5-Hydroxyeicosatetraenoic Acid Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| 5-Hydroxyeicosatetraenoic Acid Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 Vial |
| Resuspension Solution A | 1 x 300uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| Stop Solution | 1 x 6mL |
| TMB Substrate | 1 x 9mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID 5-Hydroxyeicosatetraenoic Acid
Alternative Names 5-HETE, 5(S)-HETE
CAS Number 70608-72-9
PubChem ID 5280733
Research Areas Biochemicals
Background 5-Hydroxyeicosatetraenoic Acid is a lipoxygenase metabolite of arachidonic acid and an intermediate in the pathway of leukotriene synthesis. 5-hydroxyeicosatetraenoic acid (5-HETE) is also a modulator of tubuloglomerular feedback, one of the mechanism the kidney uses to regulate glomerular filtration rate. Along with leukotriene B4, 5-HETE is often used as biomarker in evaluating eicosanoid metabolism and disease state.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.