12-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7779)
$711.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name 12-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7779)
Description 12-Hydroxyeicosatetraenoic Acid ELISA Kit (ECP7779) is a Competitive Inhibition ELISA for the quantitiation of 12-Hydroxyeicosatetraenoic Acid in a variety of aqueous sample types.
Target 12-Hydroxyeicosatetraenoic Acid
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 320.473 g·mol−1
Background Minimum Level of Detection: 5.12 ng/mL
Dynamic range of quantitation: 12.35-1,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 12.35-1,000 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| 12-Hydroxyeicosatetraenoic Acid Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| 12-Hydroxyeicosatetraenoic Acid Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 Vial |
| Resuspension Solution A | 1 x 300uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| Stop Solution | 1 x 6mL |
| TMB Substrate | 1 x 9mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID 12-Hydroxyeicosatetraenoic Acid
Alternative Names 12-HETE, 12(S)-HETE
CAS Number 71030-37-0
PubChem ID 13786989
Research Areas Biochemicals
Background 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid is a metabolite generated from arachidonic acid by 12S-type arachidonate 12-lipoxygenase in platelets. 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12(S)-HETE) may be involved in inflammation and pruritus.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.