1,25-Dihydroxyvitamin D3 ELISA Kit (ECP7846)
$700.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name 1,25-Dihydroxyvitamin D3 ELISA Kit (ECP7846)
Description 1,25-Dihydroxyvitamin D3 ELISA Kit (ECP7846) is a Competitive Inhibition ELISA for the quantitiation of 1,25-Dihydroxyvitamin D3 in a variety of aqueous sample types.
Target 1,25-Dihydroxyvitamin D3
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 416.646 g·mol−1
Background Minimum Level of Detection: 9.92 pg/mL
Dynamic range of quantitation: 24.69-2,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 24.69-2,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| 1,25-Dihydroxyvitamin D3 Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| 1,25-Dihydroxyvitamin D3 Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 x 120uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| TMB Substrate | 1 x 9mL |
| Stop Solution | 1 x 6mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID 1,25-Dihydroxyvitamin D3
Alternative Names 1,25-dihydroxycholecalciferol, 1alpha,25-dihydroxyvitamin D3, 1,25-dihydroxyvitamin D3, 1α,25-(OH)2D3, 1,25(OH)2D, Calcitriol
CAS Number 32222-06-3
PubChem ID 5280453
Research Areas Biochemicals
Background Calcitriol is a hydroxycalciol that is calcidiol in which the pro-S hydrogen of calcidiol is replaced by a hydroxy group. It is the active form of vitamin D3, produced fom calciol via hydoxylation in the liver to form calcidiol, which is subsequently oxidised in the kidney to give calcitriol. It has a role as a bone density conservation agent, an antipsoriatic, an immunomodulator, an antineoplastic agent, a calcium channel modulator, a nutraceutical, a calcium channel agonist, a metabolite, a hormone, a human metabolite and a mouse metabolite. It is a hydroxycalciol, a member of D3 vitamins and a triol.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.