Anti-δ GABA-A Receptor Antibody (56462)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,AM Mouse, Rat
SKU: 56462 Categories: , ,
Overview
Product Name Anti-δ GABA-A Receptor Antibody (56462)
Description Anti-δ GABA-A Receptor Mouse Monoclonal Antibody
Target δ GABA-A Receptor
Species Reactivity Mouse, Rat
Applications WB,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID S151-3
Isotype IgG2a
Immunogen Synthetic peptide corresponding to aa 15-34 of rat Delta GABA-A receptor (accession no. P18506).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse and rat Delta GABA-A receptor.
Target Name γ-aminobutyric acid receptor subunitδ
Target ID δ GABA-A Receptor
Uniprot ID P18506
Alternative Names GABA(A receptor subunitδ
Gene Name Gabrd
Sequence Location Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Biological Function GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Research Areas Neuroscience
Background GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families alpha, beta, g, and delta have been studied extensively; π and epsilon have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3 (56462). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56462) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.
Description Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3 (56462). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56462) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody, Clone N151/3 (56462). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody (56462) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.Immunohistochemistry and

Immunocytochemistry: use at 0.1-1ug/mL

Immunofluorescence: use at 1-10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain.
References & Data Sheet