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Anti-α B Crystallin Antibody

$378.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Human, Bovine
SKU: 11079 Categories: , ,
Overview
Product Name Anti-α B Crystallin Antibody (11079)
Description Anti-α B Crystallin Mouse Monoclonal Antibody
Target α B Crystallin
Species Reactivity Human, Bovine
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1A7.D5
Isotype IgG1
Immunogen Native alpha B crystallin
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and bovine alpha B crystallin. It does not cross-react with alpha A crystallin, beta- L crystallin, beta-H crystallin, gamma crystallin, Hsp25, Hsp27, or Hsp47 proteins.
Target Name α-crystallin B chain
Target ID α B Crystallin
Uniprot ID P02511
Alternative Names α(B-crystallin, Heat shock protein β-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Gene Name CRYAB
Gene ID 1410
Accession Number NP_001276736
Sequence Location Cytoplasm, Nucleus, Secreted, Lysosome
Biological Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity). {UniProtKB:P23927}.
Research Areas Heat Shock& Stress Proteins
Background Alpha crystallins are water-soluble lens proteins of the vertebrate eye that are related to the small heat shock protein family. Lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains proper refractive index, however it can also function as a molecular chaperone that binds to denatured proteins, keeping them in solution and maintaining the translucency of the lens. In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases. Alpha B crystallin is found in many cells and organs outside the lens and is over- expressed in cells subjected to stress conditions and in several neurological disorders.
Application Images
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~20-21 kDa is detected

ELISA: use at 1ug/ml.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Purified alpha B crystallin.
References & Data Sheet