Anti-α 2c Adrenergic Receptor Antibody (56529)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Human, Mouse
SKU: 56529 Categories: , ,
Overview
Product Name Anti-α 2c Adrenergic Receptor Antibody (56529)
Description Anti-α-2C Adrenergic Receptor Mouse Monoclonal Antibody
Target α 2c Adrenergic Receptor
Species Reactivity Human, Mouse
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S330A-80
Isotype IgG1
Immunogen Synthetic peptide corresponding to aa 442-462 (QDFRRSFFKHILFRRRRRGFQ), the cytoplasmic C-terminus, of human Alpha-2C adrenergic receptor (accession no. P18825).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse Alpha-2C adrenergic receptor.
Target Name α-2C adrenergic receptor
Target ID α 2c Adrenergic Receptor
Uniprot ID P18825
Alternative Names α-2 adrenergic receptor subtype C4,α-2C adrenoreceptor,α-2C adrenoceptor,α-2CAR
Gene Name ADRA2C
Sequence Location Cell membrane; Multi-pass membrane protein.
Biological Function Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.
Research Areas Neuroscience
Background The alpha-2C adrenergic receptor (also known as ADRA2C) controls the release of neurotransmitters from central adrenergic neurons and from sympathetic nerves in the heart. There are three subtypes of the alpha-2 adrenergic receptor: alpha-2A, alpha-2B, and alpha-2C. The human alpha-2A subtype undergoes phosphorylation and short-term desensitization, whereas the human alpha-2C subtype is not phosphorylated and does not desensitize. The human alpha-2C subtype, however, does undergo arrestin-dependent internalization. In contrast to alpha-2A and alpha-2B adrenergic receptors from several species, the human alpha-2C receptor does not appear to down-regulate in response to agonist treatment in transfected cell culture systems.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (56529). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (56529) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (56529). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (56529) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody. (D) Composite.
Description Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (56529). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (56529) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~50kDa or larger (possibly due to dimerization).

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet