Anti-α 1 GABA-A Receptor Antibody (56467)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,AM Human, Mouse, Rat
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Overview
Product Name Anti-α 1 GABA-A Receptor Antibody (56467)
Description Anti-α 1 GABA-A Receptor Mouse Monoclonal Antibody
Target α 1 GABA-A Receptor
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID S95-35
Isotype IgG2a
Immunogen Fusion protein corresponding to aa 355-394 of mouse Alpha 1 GABA-A receptor (accession no. P62812).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Alpha 1 GABA-A receptor. It does not cross-react with Alpha 2 or Alpha 3 GABA-A receptors.
Target Name γ-aminobutyric acid receptor subunitα-1
Target ID α 1 GABA-A Receptor
Uniprot ID P14867
Alternative Names GABA(A receptor subunitα-1
Gene Name GABRA1
Sequence Location Cell junction, synapse, postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane
Biological Function Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:23909897, PubMed:25489750, PubMed:29950725). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:23909897, PubMed:25489750). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (By similarity). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:23909897, PubMed:25489750). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (By similarity). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). {UniProtKB:P62812, UniProtKB:P62813, PubMed:23909897, PubMed:25489750, PubMed:29950725}.
Research Areas Neuroscience
Background GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families alpha, beta, g, and delta have been studied extensively; π and epsilon have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35 (56467). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56467) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
Description Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35 (56467). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (56467) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody, Clone N95/35 (56467). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody (56467) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.Immunohistochemistry and

Immunocytochemistry: use at 0.1-1ug/mL

Immunofluorescence: use at 1-10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain lysate.
References & Data Sheet