Specificity Any Antibody is affinity-purified on human IgE and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 4.7. Cross-reactivity with other immunoglobulins is negligible. Antibody is affinity-purified on human IgG. By immunoblotting and ELISA this antibody reacts specifically with human IgG and light chains common to other human immunoglobulins. This antibody may cros Antibody is affinity-purified on human IgM and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:protein ratio = 5.8. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on mouse IgA. Cross-reactivity with mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgE. Cross-reactivity with other immunoglobulins is negligible. Antibody is affinity-purified on mouse IgG1. Cross-reactivity with mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgG2a. Cross-reactivity with other mouse immunoglobulins is <2%. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on mouse IgG2b. Cross-reactivity with mouse IgA, IgM, IgG1, IgG2a, IgG2c, IgG3, and IgE is not detectable. Antibody is affinity-purified on mouse IgG2c. By immunoblotting and ELISA, this antibody reacts specifically with IgG2c of C57Bl/6, SJL, C57BL/10, CB20, and C57BL/6 x Balb/c mice. Cross-reactivity wi Antibody is affinity-purified on mouse IgG3. By immunoblotting and ELISA this antibody reacts specifically with mouse IgG3. Cross-reactivity with mouse IgM, IgA, IgG1, IgG2a, IgG2b, IgG2c, and IgE is Antibody is affinity-purified on mouse IgM. By immunoblotting and ELISA this antibody reacts specifically with mouse IgM. Cross-reactivity with mouse IgG subclasses and IgA is negligible. This antibo Antibody is affinity-purified on rat IgA and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgA and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5.3. This antibody may cross-react with IgA of other species. Antibody is affinity-purified on rat IgG and conjugated fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgG of other species. Antibody is affinity-purified on rat IgG1 and conjugated to fluorescein isothiocyanate. Fluorophore:antibody ratio = 5.1. This antibody may cross-react with IgG1 of other species. Antibody is affinity-purified on rat IgG2a and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 5.6. This antibody may cross-react with IgG2a of other species. Antibody is affinity-purified on rat IgG2c and conjugated to fluorescein isothiocyanate. Fluorophore:antibody ratio = 6.2. This antibody may cross-react with IgG2c of other species. Antibody is affinity-purified on rat IgM and conjugated to biotin. Biotin:antibody ratio = 4:1. This antibody may cross-react with IgM of other species. Antibody is affinity-purified on rat IgM and conjugated to fluorescein isothiocyanate (FITC). Fluorophore:antibody ratio = 7.7. This antibody may cross-react with IgM of other species. Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins. By immunoblotting and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. This antibody may cross-react with IgG of other species. By immunoblotting and ELISA this antibody reacts specifically with mouse IgG. Cross-reactivity with mouse IgA and IgM is negligible. This antibody may cross-react with IgG of other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. Reactivity with non- immunoglobulin serum proteins By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. Reactivity with other non-immunoglobulin serum pro By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross-reactivity with IgG, IgM and light chains is less than 0.1%. This antibody may cross-react with IgA from oth By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross-reactivity with IgG, IgM and light chains is less than 0.1%. This antibody may cross-react with IgA of other By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgE. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross-reactivity with other immunoglobulins and light chains is less than 0.1%. This antibody may cross-react with By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. Reactivity with non- immunoglobulin serum proteins is By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG- Fc. Cross-reactivity with IgA and IgM is negligible. This antibody may cross-react with IgG from other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. Reactivity with non- immunoglobulin serum proteins i By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG- Fc. Reactivity with Fab fragments is not detected. This antibody may cross-react with IgG from other species. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG- Fc. Reactivity with rabbit Fab fragments is not detected. This antibody may cross-react with IgG from other speci