Anti-Mre11 Antibody (56211)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IHC-P,IP,ELISA,Functional Assay,PLA Human
SKU: 56211 Categories: , ,
Overview
Product Name Anti-Mre11 Antibody (56211)
Description Anti-Mre11 Mouse Monoclonal Antibody
Target Mre11
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IP,ELISA,Functional Assay,PLA
Host Mouse
Clonality Monoclonal
Clone ID 12D7
Isotype IgG1
Immunogen GST fusion protein corresponding to aa 182-582 of Mre11.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Mre11.
Target Name Double-strand break repair protein MRE11
Target ID Mre11
Uniprot ID P49959
Alternative Names EC 3.1.-.-, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A
Gene Name MRE11
Sequence Location Nucleus, Chromosome, telomere, Chromosome
Biological Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}.
Research Areas Cancer research
Application Images
Description Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Description Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.
A. 30 ug 293T whole cell extract
B. 30 ug whole cell extract of human Mre11-transfected 293T cells
7.5% SDS-PAGE
Mre11 antibody [12D7] (56211) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Mre11 stained by Mre11 antibody [12D7] (56211) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 um.
Description Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
Description Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
Mre11 stained by Mre11 antibody [12D7] (56211) diluted at 1:300.
Antigen Retrieval: EDTA buffer, 20 min
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts.

Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet