Immunochemistry Services

QED offers a wide variety of immunochemistry services that are designed to provide you with an antibody product that is in the perfect form for your applications. All immunochemistry services are done to cGMP standards.

Antibody Purification

QED will purify antibody from serum, ascites fluid, or culture medium using a variety of purification procedures. These procedures include ammonium sulfate or PEG precipitation, Protein-G chromatography, and affinity chromatography.

Ammonium sulfate precipitation is a first step in separating antibodies from other serum or ascites proteins. It is useful for preparing antibodies for further purification by ion exchange chromatography, or when you do not need a highly purified antibody preparation. PEG precipitation is used to purify mouse IgM and IgG3 antibodies since they bind poorly to Protein-A and Protein-G.

Protein-G chromatography yields pure antibody from serum, ascites fluid, or culture medium without an initial preparatory step such as ammonium sulfate precipitation. Purification on these media is rapid and can be applied to small and large sample volumes.

Affinity chromatography is used with polyclonal antiserum in which specific antibody comprises only 5-10% of the total immunoglobulin. Affinity chromatography selectively purifies the specific antibody of interest from the remaining antibodies in the antiserum.

Antibody-Reporter Conjugations

Antibody-Reporter Conjugations QED is experienced in enzyme, biotin, and fluorophore conjugation techniques. By conjugating these labels to your antibody, you can create a primary immunoreagent. Directly labeling your primary antibody allows you to eliminate steps in your assays, saving time and money. QED can assist you in determining which reporter molecule is best for your application.

Hapten-Carrier Conjugations

Small haptens, or poorly reactive antigens, are often made more antigenic when conjugated to carrier proteins, such as keyhole limpet hemocyanin. QED will assist you in determining whether conjugation is appropriate for your antigen, and which carrier protein is best for your application

ELISA Development and Testing

ELISAs (Enzyme Linked Immunosorbent Assays) are used to measure the specific binding of an antibody to an antigen. This is a rapid and sensitive method for evaluating large numbers of monoclonal or polyclonal antibodies at low cost. QED scientists have years of experience developing ELISAs for our clients and for our own in-house antibodies and we are pleased to share that experience with you. Once an ELISA has been developed for you, we will transfer the ELISA protocol to you and, if you wish, apply that ELISA to testing of your antibody samples.

Peptide Synthesis

QED works with expert peptide chemists who will synthesize peptides or multiple antigenic peptide (MAP) configurations and conjugate peptides to your specifications. Contact a QED representative for a customized price quote.