|Ammonium Sulfate Precipitation||Polyethylene Glycol (PEG) Precipitation|
Protein-G chromatography yields pure antibody from serum, ascites fluid, or culture medium without an initial preparatory step such as ammonium sulfate precipitation. Purification on these media is rapid and can be applied to small and large sample volumes.
|$250.00 per 1-25ml||>25ml @ $10.00/ml|
Culture Supernatant Purification
Affinity chromatography is used with polyclonal antiserum in which specific antibody comprises only 5-10% of the total immunoglobulin. Affinity chromatography selectively purifies the specific antibody of interest from the remaining antibodies in the antiserum.
|Affinity Column||$595.00 per column||Requires ~10mg antigen/column|
|Affinity Purification||$250.00 per 1-25ml||>25ml @ $10.00/ml|
A single-step, primary immunoreagent can be made by conjugating various labels directly to antibodies. QED offers biotin, horseradish peroxidase (HRP), FITC, alkaline phosphatase, and phycoerythrin labeling services. Prices for these services vary with the type of label and quantity of antibody to be labeled. Please contact your QED representative for a customized price quotation at 800-929-2114 / 858-675-2405 or email@example.com.
Small haptens, or poorly reactive antigens, are often made more antigenic when conjugated to carrier proteins, such as keyhole limpet hemocyanin. QED will assist you in determining whether conjugation is appropriate for your antigen, and which carrier protein is best for your application
ELISAs (Enzyme Linked Immunosorbent Assays) are used to measure the specific binding of an antibody to an antigen. This is a rapid and sensitive method for evaluating large numbers of monoclonal or polyclonal antibodies at low cost. QED scientists have years of experience developing ELISAs for our clients and for our own in-house antibodies and we are pleased to share that experience with you. Once an ELISA has been developed for you, we will transfer the ELISA protocol to you and, if you wish, apply that ELISA to testing of your antibody samples.
|ELISA development||$2,500 per assay|
|ELISA testing of antibodies||$100 per plate|
QED works with expert peptide chemists who will synthesize peptides or multiple antigenic peptide (MAP) configurations and conjugate peptides to your specifications. Contact a QED representative for a customized price quote.